As mentioned above, CTX-induced muscle injury is characterized by the influx of calcium, which might have contributed to the upregulation of the Akt-mTOR axis. In the present study, the expression of the upstream mTORC1 intermediates, e.g. p-Akt and p-mTOR, peaked 5 days following CTX injury compared to … See more We demonstrated that the slower type IIa fibers were more vulnerable to CTX-injection and that the formation of primarily fast muscle fibers preceded the slow fibers in the TA, i.e. slow type IIa fibers were decreased at d2 and … See more Our data reveal that CTX severely impacts muscle histology and molecular signaling. To better understand the regeneration process, the potential physiological meaning(s) of the … See more In their response upon injury, molecular targets exhibit differential expression patterns, reflecting their unique functioning during the … See more Besides the upregulation of a muscle stress response, muscle regeneration is also characterized by an anabolic response (Ge et al. 2009). mTORC1 signaling, which is the key regulator of protein synthesis, … See more WebMar 18, 2024 · This protocol includes cardiotoxin induced muscle injury and also describes isolation of satellite cells from skeletal muscle tissues of mice. This protocol can be used …
Early ultrastructural events of skeletal muscle damage following ...
WebNov 1, 2015 · However, CTX injection induced greater muscle degeneration than glycerol injection; vacuolated myofibers could be seen after CTX injection. Small regenerating … WebJul 8, 2024 · To examine CNN3 expression during muscle regeneration, we injected CTX into the hindlimb muscles to induce muscle injury and regeneration (Singh et al., 2014; ... Our model of CTX-induced injury in skeletal muscle revealed that the general trend of CNN3 gene expression was similar to that of Pax7 and MyoD, suggesting that CNN3 … shuckers ridgeland ms facebook
Comparative Study of Injury Models for Studying Muscle ... - PLOS
WebHere, we describe our experience using cardiotoxin as a potent myonecrotic agent to study skeletal muscle regeneration. We provide a detailed protocol to examine skeletal … WebApr 11, 2024 · Background Chemotherapeutic drugs, particularly alkylating cytotoxics such as cyclophosphamide (CTX), play an important role to induce premature ovarian failure (POF). Hormone replacement therapy (HRT) is a widely used treatment to improve hormone secretion. However, the long-term HRT increases the risk of breast cancer and … WebMar 24, 2014 · C-telopeptide fragments of type II collagen (CTX-II) are created during articular cartilage breakdown and CTX-II is considered useful as a biomarker of … the other clinic