WebAfter being incubated with Dynabeads MyOneTM Streptavidin C1 beads (Thermo Fisher, 65002) for 1 hour at room temperature, cDNA Libraries were generated using KAPA Hyper Prep Kit (Kapa Biosystems, kk8505). After adaptor ligation, the libraries were amplified by 8 cycles of PCR using QP2 primer and short universal primer. The libraries were ... WebDynabeads MyOne Streptavidin C1 The following streptavidin beads led to reduced assay performance and are not recommended: PureProteome Streptavidin Magnetic Beads (Sigma) Streptavidin Magnetic Beads (NEB) Products: Targeted Gene Expression
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WebMay 14, 2024 · Dynabeads MyOne Streptavidin C1. カルボキシル基活性化ビーズをベースに作られた、親水性の表面を持つ粒径1.0 μmの超常磁性高分子ポリマービーズにストレプトアビジンを結合させたビーズです … WebMar 16, 2024 · Lanes 1–4: Dynabeads MyOne Streptavidin C1 eluates; Lanes 5–8: Sera-Mag bead eluates (10 mM, 20 mM, 75 mM, and 150 mM NaOH, respectively); Lane 9: purified streptavidin [exists as a tetramer with a combined molecular weight of approximately 60 kDa that can break into trimers (∼45 kDa, * ), dimers (∼30 KDa, * 1) or … chinese dollars to pounds uk
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http://plant-plasticity.github.io/resources/T-DNA%20mapping%20protocol%20website%20version.pdf WebDynabeads™ MyOne™ Streptavidin C1 Beads are uniform, 1.0 μm dia. superparamagnetic beads with a streptavidin monolayer covalently coupled to the hydrophilic bead surface. … Webapproximately 4× (for Dynabeads) and 2× (for Sera-Mag beads) less dsDNA contamination compared with 75 mM and 150 mM NaOH elutions (Figure 1A, Lanes 4 and 5 for Dynabeads and Lanes 8 and 9 for Sera-Mag beads). Overall, 10 mM and 20 mM NaOH elutions from Dynabeads MyOne Streptavidin C1 had 1.6× less dsDNA contamination and grand haven community palm coast fl