E2a antibody

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August undefined, 2024

WebImmunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21242-1-AP (E2A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Jurkat cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. WebAll lanes : Anti-TCF3 / E2A antibody - N-terminal (ab229605) at 1/2500 dilution Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract Lane 2 : …

E2A Polyclonal Antibody (21242-1-AP) - Thermo Fisher …

WebE2A Antibody (D-7) is a mouse monoclonal IgG 1 κ, cited in 1 publications, provided at 200 µg/ml; raised against amino acids 1-649 of E2A of human origin; recommended for detection of E2A isoforms E12 and E47 of human origin by WB, IP, IF, IHC(P) and ELISA; TransCruz reagent for ChIP application (sc-133074 X, 200 µg/0.1 ml) WebApr 8, 2024 · I ChIP assays were performed in A549 cells using a specific E2A antibody (anti-E47) or a control rabbit normal IgG (anti-IgG) and primers specific for P1, P2, and P3. A randomly segment (−40 to ... portable cooking kit

E2A CRISPR Knockout and Activation Products (h) - SCBT

Webwith antibodies to the Myc-tag (9E10), E12/47 (E2A.E12), or phos-pho E2A pT355 (Bethyl Laboratories) as indicated. Generation of Stable Cell Lines Expressing MyoD-ER and North-ern Blot Analysis The MDER-SN construct containing both the chimeric MyoD-ER cDNA and neo selectable marker gene (Hollenberg et al., 1993) was WebNational Center for Biotechnology Information WebB-lymphocytes can modify their immunoglobulin (Ig) genes to generate specific antibodies with a new isotype and enhanced affinity against an antigen. Activation-induced cytidine deaminase (AID), which is positively regulated by the transcription factor E2A, is the key enzyme that initiates these processes by deaminating cytosine to uracil in Ig ... portable cooking oven

Mouse TCF-3/E2A Antibody AF7650: R&D Systems

Transcription Factor E2A - an overview ScienceDirect Topics

WebE2A is a member of the E-protein family of transcription factors, a subclass of basic helix-loop-helix (bHLH) proteins that bind specifically to E-box … WebE2A Antibodies. Santa Cruz Biotechnology, Inc. offers a broad range of E2A antibodies. Select E2A antibodies from monoclonal antibodies listed below. View detailed E2A antibody specifications by linking to the specific product blocks. Select appropriate E2A antibodies for your research by isotype, epitope, applications and species reactivity. irrigability classificationWebDuring the transition of CLP into B-cell lineages via pre–pro-B cells, transcription factors E2A, early B cell factor 1 (EBF1), and PAX5 play crucial combined roles in a hierarchical … portable cooking station

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E2a antibody

Flexible ordering of antibody class switch and V(D)J joining …

WebThere are no reviews for TCF-3/E2A Antibody (NBP2-67437). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen; Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen; Submit a Review. Product General Protocols ... WebE12, E47, E2-2, and HEB are each present in B cells. The presence of many different HLH proteins raises the question of which of the HLH proteins actually binds the Ig enhancer elements in B cells. Using monoclonal antibodies specific for both E2A and E2-2, we show that both E2-2 and E2A polypeptides are present in B-cell-specific Ig enhancer ...

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WebTCF-3/E2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44061) at 10 µg/mL for 3 hours at room temperature. Cells were … WebWestern Blot: TCF3/E2A Antibody [NBP2-20585] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:2000. Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585] - Jurkat whole cell extract A. 35 ug Jurkat whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of TCF3 protein by 4 ug TCF3 ...

WebJan 25, 2015 · Linking the heavy chain (HC) and light chain (LC) genes required for monoclonal antibodies (mAb) production on a single cassette using 2A peptides allows control of LC and HC ratio and reduces non-expressing cells. Four 2A peptides derived from the foot-and-mouth disease virus (F2A), equine rhinitis A virus (E2A), porcine … WebThe translocation results in the transcription of a unique chimeric protein E2A-Pbx1 with an amino acid sequences not present on E2A or Pbx alone. E2A-Pbx1 is found only in cells with the t (1,19) translocation. It is detectable in the nucleus of 697 cell line (a pre-B cell line). E2A-Pbx1 is considered to be an oncoprotein, however, the ...

WebTCF-3 (Transcription factor 3; also Transcription factor A1 and E2A/alpha plus E12/E47) is a 68-75 kDa nuclear class I member of the bHLH family of molecules. It should not be confused with TCF-3, otherwise known as TCF7-L1, an epidermis-associated beta -catenin regulator. TCF-3 is ubiquitously expressed, and generally serves as a ... WebGeneral notes. ab251582 is the carrier-free version of ab224638. Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency. This conjugation-ready format is designed for use with ...

WebPolyclonal Antibody for studying E2A. Cited in 7 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, E2A Antibody (CST #4865) is ready to ship.

WebMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly112 of human E2A protein. Background E2A … irrifix tabletWebTCF-3/E2A was detected in immersion fixed frozen sections of E9.5 mouse embryo using Rat Anti-Mouse TCF-3/E2A Monoclonal Antibody (Catalog # MAB7650) at 10 µg/mL overnight at 4 °C. Tissue was stained using the … portable cooking stove butaneWebGeneral description. Anti-E2A Antibody detects endogenous levels of total E2A protein. The E2A gene is mapped to human chromosome 19p13.3. [ 1] The protein encoded is a … portable cooking tableWebE2A Antibody (V-18) is a rabbit polyclonal IgG; 200 µg/ml. epitope mapping at the C-terminus of E2A of human origin. Discontinued polyclonal antibody. See product citations (38) irrigador oral mornwell d52Webexpression in an E2A / pre-pro-B cell line, in pro-B cells isolated from Rag1-orMb1-deficient mice, and in a panel of Abelson transformed pro-B-cell lines (Rag-deficient ... combination, and antibody production by immature/transitional 1 B cells. J Immunol 178: 3593–3601. von Boehmer H, Melchers F. 2010. Checkpoints in lymphocyte develop- portable cooking rangeWebTCF-3/E2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44061) at 10 µg/mL … portable cooktop walmart -inductionWeb实验操作步骤： Anti-AQP10 Polyclonal Antibody价格是用于测定细胞增殖或毒性实验中活细胞数目的一种高灵敏度，无放射性的比色检测法。CCK法应用非常广泛，如药物筛选、细胞增殖测定、细胞毒性测定、肿瘤药敏试验以及生物因子的活性检测等。下面以engreen的C portable cooking stove electric