WebATCC geobacter Geobacter, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … WebSir2-domain associated short prokaryotic Argonautes provide defence against invading mobile genetic elements through NAD+ depletion
Short prokaryotic Argonautes provide defence against …
WebGsSir2/Ago-ssRNA complex bound to complementary ssDNA target ~140-fold better than apo-GsSir2/Ago bound pre-annealed gRNA/tDNA heteroduplex (Fig. 4B, Table 1). On … WebOct 3, 2024 · The GsSir2 protein contains a His 6-Tag at its N terminus. The mutants DSR2 (N133A) and DSR2 (H171A) were constructed using the Q5 Site-directed Mutagenesis … batmani tagasitulek
(PDF) Sir2-domain associated short prokaryotic …
WebThe binary GsSir2/Ago-gRNA complex (0.5 μM) was incubated with NAD⁺ (50 μM) for 1 h at 37 °C in the presence of the complementary DNA target (0.5 μM). NAD⁺ depletion and … Web1 hour ago · The mechanism, guide/target preferences and function of short pAgos is an emerging topic in the Argonaute field, as evidenced by recent characterization of … WebThe GsSir2/Ago complex presumably recognizes invading plasmid or phage DNA and activates the Sir2 subunit, which triggers endogenous NAD+ depletion and cell death, … test gravidanza 8 post iui