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Pmcherry-gfp-lc3

WebmCherry-GFP-LC3 Use Lentiviral Tags Expression Mutation Promoter Availability Academic Institutions and Nonprofits only Enlarge pDEST-CMV mCherry-GFP-LC3B WT Plasmid #123230 Purpose Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter. Depositor Webmcherry-GFP-Lc3 reporter cells enable flow cytometric quantification and sorting of cells based on autophagic flux. (A) Quantification of autophagic flux by flow cytometry using …

Addgene: pmCherry-Gal3

WebpmCherry-EGFP-LC3b. Catalog No. PVT10398 Packing 2ug . pmCherry-EGFP-LC3b Information Function ORF expression plasmid. Promoter: CMV. Replicator: pUC ori, F1 ori, … WebApr 12, 2024 · First, we found that red fluorescent protein (RFP)-tagged LC3 colocalized with green fluorescent protein (GFP)-tagged BH3 interacting domain death agonist (BID-GFP), a mitochondrial marker protein in HPIV3-infected cells, and we used carbonyl cyanide 3-chlorophenyl hydrazine (CCCP)-induced mitophagy as a positive control ( Figure 1 A), … oldcastle precast chandler az https://letmycookingtalk.com

pCMV-GFP-LC3B(pCMV-GFP-LC3B质粒)(D2815-1μg)

WebSep 13, 2009 · To generate MDCK cells stably expressing GFP–LC3 and p62-3×Myc, ... MDCK/pmCherry–LC3 cells were seeded at 4 × 10 4 cells ml −1 on 35-mm glass bottom dishes and cultured for 4 days at 37 °C. WebPlasmid pmCherry-Gal3 from Dr. Hemmo Meyer's lab contains the insert LGALS3 and is published in EMBO J. 2016 Oct 17. pii: e201695148. This plasmid is available through … WebApr 11, 2024 · LC3 and SQSTM1 expression were measured by western blot. GAPDH was used as internal control. (C and D) MIN6 cells were transfected with mRFP-GFP-LC3 adenovirus and the dots per cell were quantified in D (n = 10). Scale bar: 20 μm. (E and F) INS1 cells were transfected with mRFP-GFP-LC3 and the dots per cell were quantified in F … oldcastle primary school bridgend

LC3双荧光自噬慢病毒的应用（上篇）——实验原理及病毒 …

Category:mcherry-GFP-Lc3 reporter cells enable flow cytometric quantification

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Pmcherry-gfp-lc3

Bone Mesenchymal Stem Cell-Derived Extracellular Vesicles ... - Hindawi

WebFUW mCherry-GFP-LC3 (Plasmid #110060) Print Enlarge View all sequences Purpose To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes … WebMost ATG factors but not LC3 homologs detach from the isolation membranes before completion of autophagosomes. These isolation membrane markers are localized on the …

Pmcherry-gfp-lc3

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WebJan 12, 2024 · Autophagy Formation Traced by mRFP-GFP-LC3 Double Fluorescence Assay. Chondrocytes were cultured on coverslips for 24 h and then transfected with pmCherry-GFP-LC3 plasmid utilizing Lipofectamine 2000 reagent (Invitrogen) for 24 h. Next, the cells were fixed with 4% paraformaldehyde and observed with a confocal fluorescence … WebPlasmid pCDH-EF1a-mCherry-EGFP-LC3B from Dr. Sang-Hun Lee's lab contains the insert mCherry-GFP-LC3 and is published in Sci Adv. 2024 Feb 17;7(8). pii: 7/8/eabb1540. doi: 10.1126/sciadv.abb1540. Print 2024 Feb. This plasmid is available through Addgene.

WebJun 2, 2011 · A tandem fusion of mCherry and GFP is fused to LC3B (one of the several members of the mammalian LC3 family) to make a pH-sensitive sensor that is used to monitor autophagy in live cells. The GFP tag is acid-sensitive while the mCherry tag is acid-insensitive. The double tagged LC3 can be used to label autophagosomes, amphisomes … WebDec 27, 2024 · LC3（light chain 3）简称于MAP1LC3 (microtubule-associated proteins light chain 3)，是目前公认的自噬标记物。哺乳动物中的LC3与酵母中的自噬相关蛋 …

Web·LC3荧光双标腺病毒系统（Ad-mCherry-GFP-LC3，GCD0165968): 山东大学附属山东省耳鼻喉医院王海波教授团队与东南大学柴人杰教授合作在自噬领域专业期刊《Autophagy》上发表了题为“PRDX1 activates autophagy via the PTEN-AKT signaling pathway to protect against cisplatin-induced spiral ganglion neuron damage”的文章，阐明了自噬保护SGN免受顺铂损 … Web(A) Quantification of autophagic flux by flow cytometry using reporter cells stably expressing mCherry-GFP-LC3. Using the ratio of mCherry to GFP, autophagic flux can be easily and …

WebpCMV-GFP-LC3B是碧云天自行研发的在哺乳动物细胞中表达GFP标签的人源LC3B 融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染细胞后能够在靶细胞中高效表达绿 …

WebpDEST-CMV mCherry-GFP-LC3B WT (Plasmid #123230) Print Enlarge View all sequences Purpose Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent … my own environmental wellness strategiesWebWe detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations … my own expenseWebTransgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from … my own eyes ytWebView publication. Transfection of the mCherry-GFP-LC3 plasmid into IEC-18 cells and quantification of the autophagic puncta by confocal microscopy. (A) IEC-18 cells were cultured under normal ... oldcastle rockwood tennesseeWebMar 17, 2024 · GFP-LC3 has been used as a fluorescent marker for autophagosomes (AP) ... pEGFP-LC3 (Addgene 24920) was double digested with AfeI (blunt) and BamHI, yielding the 1.2 kb EGFP-LC3 segment. This segment was subcloned into pmCherry-C1 (Addgene 632524), which was opened by the digestion of SmaI (blunt) and BamHI. Experimental … oldcastle rockwood tnWebJul 22, 2024 · Using a reporter construct expressing a fusion protein of GFP, LC3, RFP, and a non-cleavable variant of LC3B, LC3BΔG, this system allows, similarly to the double-labeled LC3B, monitoring of ... oldcastle retaining wall block 3x8x5WebNov 7, 2015 · Thus, GFP-LC3puncta itself cannot serve sufficientevidence enhancedautophagy. However, shortcomingmay tandemfluorescent-tagged LC3 (tfLC3), … oldcastle tcr 225