005688 FVB-Tg(Rag2-EGFP)1Mnz/J These NG-BAC transgenic mice express enhanced green fluorescent protein (EGFP) directed by the Rag2 promoter, and may be used in studies of B and T cell development as EGFP fluorescence reflects Rag2 protein expression. WebAug 13, 1999 · The regulation of RAG1 and RAG2 was investigated in vivo with bacterial artificial chromosome (BAC) transgenes containing a fluorescent indicator. Coordinate …

RAG2:GFP knockin mice reveal novel aspects of RAG2

WebFeb 25, 2010 · (A) Rag2 −/− γ c −/− male mice received a transplant subcutaneously in the left flank of MEC1 cells with or without GFP expression (10 × 10 6 cells/mouse; n = 4, repeated twice), showing no significant difference in tumor engraftment. Tumor size was evaluated by measuring perpendicular diameters by a caliper. WebRFP mice splenocytes stained with B220 APC and CD8 APC (down) ( B ) Representative FACS plots of post-depletion samples composed of a mix of B220-, CD8-, IL18R- depleted Foxp3. GFP splenocytes and B220-, CD8- depleted Foxp3. RFP splenocytes. Numbers indicate the percentage of cells within each gate. Techniques Used: Staining Figure … mahjong free games xbox

Rag2 Targeted Allele Detail MGI Mouse …

WebTCRβ + CD4 + and CD8 + T cells from spleen, LNs, and PBCs of Rag2GFPSrsf1fl/flLckCre/+ and littermate Rag2GFP (as control) mice were measured by flow cytometry. Representative histograms show the percentages of GFP + cells from indicated populations. WebThese grafts are not rejected in this mouse strain because the Rag2 −/− γ c −/− mice are immunodeficient, lacking NK, T and B cells. The recovered FTOC-T cells were subcutaneously infused around the skin graft ( Figure 5D ) 4 weeks posttransplantation to determine if the T cells recognized and responded to the allogenic MRL skin grafts. WebGeneration of RAG2 knockout, GFP knock-in (RAG2-GFP) mice. (A) Design of the RAG2 knockout, GFP knock-in targeting construct. The genomic restriction endonuclease map … mahjong freegames.ws