WebAbstract. The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a … WebMay 16, 2024 · Scrape loading can measure dye transfer in multiple cells almost simultaneously, and it is the only visual method available to assess the overall GJIC of a …
Assay for molecular transport across gap junction channels in one …
WebJan 20, 2024 · The commonly used technique for in vitro assessment of GJIC is so-called scrape loading-dye transfer assay (SL-DT) using a membrane-impermeable and gap … WebFeb 1, 1987 · It employs scrape-loading to introduce a low molecular weight (MW) fluorescent dye, Lucifer yellow CH (MW 457.2) into cells in culture and allows the … mild septal thickening
Applicability of Scrape Loading-Dye Transfer Assay for Non …
WebThe GJIC was investigated by a scrape-loading dye transfer assay. Expression of Cx43 and K3 keratin as well as BrdU-retaining nuclei were also ana-lyzed after xenotransplantation in nude mice. Results: Limbal epithelium showed mean±SD 12.4% ±14.5%positiveunitsof Cx43expressionandalowBrdU WebGap junction communication, assessed with a scrape-loading dye transfer assay, was significantly increased in direct transmembrane co-cultures … WebFeb 7, 2024 · e Images obtained from the scrape loading (SL)/dye transfer (DT) assay after application of lucifer yellow CH dilithium salt (LY, MW 457), which transfers via the functional gap junction channels ... mild seven charcoal filter cigarettes