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Scrape-loading dye transfer assay

WebAbstract. The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a … WebMay 16, 2024 · Scrape loading can measure dye transfer in multiple cells almost simultaneously, and it is the only visual method available to assess the overall GJIC of a …

Assay for molecular transport across gap junction channels in one …

WebJan 20, 2024 · The commonly used technique for in vitro assessment of GJIC is so-called scrape loading-dye transfer assay (SL-DT) using a membrane-impermeable and gap … WebFeb 1, 1987 · It employs scrape-loading to introduce a low molecular weight (MW) fluorescent dye, Lucifer yellow CH (MW 457.2) into cells in culture and allows the … mild septal thickening https://letmycookingtalk.com

Applicability of Scrape Loading-Dye Transfer Assay for Non …

WebThe GJIC was investigated by a scrape-loading dye transfer assay. Expression of Cx43 and K3 keratin as well as BrdU-retaining nuclei were also ana-lyzed after xenotransplantation in nude mice. Results: Limbal epithelium showed mean±SD 12.4% ±14.5%positiveunitsof Cx43expressionandalowBrdU WebGap junction communication, assessed with a scrape-loading dye transfer assay, was significantly increased in direct transmembrane co-cultures … WebFeb 7, 2024 · e Images obtained from the scrape loading (SL)/dye transfer (DT) assay after application of lucifer yellow CH dilithium salt (LY, MW 457), which transfers via the functional gap junction channels ... mild seven charcoal filter cigarettes

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Scrape-loading dye transfer assay

Changes in cell morphology affect the quantification of …

WebSep 3, 2024 · MC3T3-E1 cell line were cultured in a 25 cm2flask containing 3-4 ml α-MEM with 10% fetal bovine serum at 37 °C in a humidified atmosphere of 5% CO2in the air. FGF7 treatment Both primary osteoblasts and MC3T3-E1 cell line were seeded onto six-well plates at 5 × 105cells per well (85%~95% confluence). WebVoltage is an important parameter that regulates the open probability of both intercellular channels (gap junctions) and undocked hemichannels formed by members of the connexin gene family. All ...

Scrape-loading dye transfer assay

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WebMar 7, 2024 · Scrape-loading dye transfer in vitro. Gap junction-mediated intercellular coupling was evaluated by transfer of lucifer yellow dye in a scrape-loading assay as described previously with minor modifications . Briefly, astrocytes were seeded on 12-well culture plates and incubated at 37 °C and 5% humidified atmosphere.

WebJan 20, 2024 · The commonly used technique for in vitro assessment of GJIC is so-called scrape loading-dye transfer assay (SL-DT) using a membrane-impermeable and gap … WebFigure 7 Utility of the scrape loading/dye transfer assay to detect a toxin/toxicant s ability to modulate gap junctional intercellular communication. The last two images (6ngmr TPA) illustrate both the epifluorescent and phase images, respectively, of the same cells to show that the complete inhibition of GJIC was not associated with the death ...

WebFeb 7, 2024 · e Images obtained from the scrape loading (SL)/dye transfer (DT) assay after application of lucifer yellow CH dilithium salt (LY, MW 457), which transfers via the … WebJul 1, 2008 · The scrape loading and dye transfer (SL/DT) method is a functional assay widely used to evaluate the level of the intercellular communication via gap junction. Initially, the evaluation of the degree of communication was done by counting the rows of fluorescent cells from the scrape.

WebSep 18, 2014 · Scrape-loading/dye transfer assay for gap junctional intercellular communication Solutions needed: HBSS + 1% BSA (50 ml; 0.5 g BSA/HBSS) PBS + 1% …

WebA scrape loading/dye transfer assay was used to assess the effects of the chemicals on GJIC. In this assay, a scratch is made in a confluent cell layer in the presence of a Lucifer … new year\u0027s eve party bagsWebFeb 28, 2011 · An innovative adaptation of traditional, fluorescence microscopy-based scrape loading-dye transfer (SL-DT) assay is presented, which has been optimized to simultaneously evaluate GJIC, cell density and viability, which could be included into in vitro screening and assessment of pharmacologically and toxicologically relevant compounds. … new year\u0027s eve party drinksWebMar 1, 1987 · Scrape loading/dye transfer method (SL/DT) was used to analyse gap junction activity of cells, as previously reported [29]. Myocytes and macrophages were seeded in … new year\u0027s eve party edmonton 2023