WebOct 21, 2013 · Design your PCR primers according to the following guidelines suggested by IDT scientists: Melting temperature (Tm): The optimal melting temperature of the primers is 60–64°C, with an ideal temperature of 62°C, which is based on typical cycling and reaction conditions and the optimum temperature for PCR enzyme function. WebOne type of dye, such as ethidium bromide, intercalates in between bases inside the DNA, and. The secondary structure of DNA is often B-DNA, which is a double helix that contains a minor groove and a major groove. When dying DNA molecules for DNA laboratory techniques, there are two general types of dyes that specifically bind to DNA to make it ...
SYBR® Green Fluorescent DNA Stain - Jena Bioscience
WebDye concentrations were calculated based on the stock solution concen-trations provided by the supplier, with the exception of EvaGreen, the molar concentration of which could not be calculated as the dye’s chemical structure is not publicly available. For SYBR Green I solution, 1× equals 2 µM (25). Stock solutions of dyes (200 µM; WebNov 9, 2007 · Extinction coefficient of SYBR Green I. Zipper, et al. elegantly revealed the structure of SYBR Green I using a combination of analytical techniques [-1 cm-1 value reported by Zipper, et al. and dramatically different from the extinction coefficients of dyes in the same class. For example, TO-PRO has an extinction of about 60,000 (Haugland, R.P ... speedway jobs pay
QuantiTect SYBR Green RT-PCR Kit - QIAGEN
WebDec 1, 2024 · The commercial dye, SYBR Green II and MeS, which have R–S arm instead of -NMe 2, differ from the above as the R–S group is co-planar with the main skeleton and the phenyl ring twist angle is closer to 90° (MeS 79.3° and SYBR Green II 88.1°). The overall structure of these dyes is very similar (see the VDW radii plots in the SI, Fig. S16 ... WebJun 1, 2024 · While the structures of SYBR Green I and PicoGreen are known from literature, the structure of SYBR Green II was here elucidated through X-ray crystallography, together with MeS 1 (Fig. 2 a and b). The only difference in structure between the MeS dye 1 and SYBR Green II is the different pendant arm on the molecule. Comparing the crystal … WebFeb 2, 2024 · (D) BMDC incubated with SYBR-green pre-stained phage (green) (bottom), or stained directly with SYBR green (top) fixed and stained with Alexa Fluor 555 Phalloidin for cytoplasm staining (red). (E) BMDCs incubated with SYBR green pre-stained phage and subsequently stained with Alexa Fluor 680 Phallodin (blue) or a Histone 2B-RFP dye (red). speedway jeffersonville ohio